{"id":191,"date":"2023-03-11T17:11:01","date_gmt":"2023-03-11T17:11:01","guid":{"rendered":"https:\/\/arcaid-h2020.eu\/?page_id=191"},"modified":"2023-03-17T14:09:07","modified_gmt":"2023-03-17T14:09:07","slug":"melis_longbio","status":"publish","type":"page","link":"https:\/\/arcaid-h2020.eu\/?page_id=191","title":{"rendered":"Melis_LongBio"},"content":{"rendered":"

\"\"Melis Asal <\/span><\/strong><\/span><\/p>\n

ESR19- <\/span><\/strong><\/span>A human gut and -lymphatics on chip to model functional immune surveillance and activation; development of immune competent gut on chip model <\/strong><\/span><\/p>\n

Education<\/strong><\/span><\/p>\n

2017-2020 Master of Science<\/strong> <\/span><\/p>\n

The Dokuz Eylul University, Izmir International Biomedicine and Genome Institute, Department of Molecular Biology and Genetics, Therapeutic Bioengineering Group, Izmir, <\/span>Thesis title: Development of Lacrimal Gland from Human Induced Pluripotent Stem Cells Using Tissue Engineering Approaches<\/span> <\/span><\/span><\/span><\/span><\/p>\n

My thesis focuses on differentiation of hiPSCs in a microfluidic system in 3D to offer a treatment method for dry eye syndrome. In order to achieve this, I have developed the strategy to differentiate hiPSCs into cells that are capable of developing the lacrimal gland by following different methods and making modifications. I have monitored the differentiation by checking the expression of markers through ICC, IHC and qPCR. I am currently working on transferring the maturation process into a microfluidic system. <\/em><\/span><\/p>\n

Student Supervision: Effect of potential therapeutic agent on breast cancer cells under dynamic conditions<\/span><\/p>\n

I supervised a high school project that took place in our lab where we investigated the apoptotic effect of CAPE in a breast cancer cell line by objecting the cells to flow. I contributed to the experimental setup of this project and showed the students the principles of cell culture, microfluidic chip fabrication and cell staining studies. <\/em><\/span><\/p>\n

2013-2017     Bachelor of Engineering (High Honor Student)<\/strong><\/span><\/p>\n

Manisa Celal Bayar University, Faculty of Engineering, Department of Bioengineering, Manisa, Turkey<\/span><\/p>\n

Title: Independent research activity (Funded by The Scientific and Technological Research Council of Turkey: The Effect of Mesenchymal Stem Cells and Thyme Oil in Wound Healing <\/span><\/p>\n

This project aimed investigating the use of thyme oil to increase the therapeutic effect of MSC in wound healing. I created wound models on Wistar rats and treated with different combinations of rat adipose mesenchymal stem cells, thyme oil and Madecassol. The synergistic effect of the combination was analyzed morphologically, histologically and immunohistochemically.<\/em><\/span><\/p>\n

Bachelor\u2019s Thesis: The Effect of Different Seed Extracts on Microbial Quality of Meat Products <\/span><\/p>\n

The aim of this project was to increase the microbial safety of meat products by incorporating plant extracts. I assessed the antibacterial effects of hot ethanol extracts of dog rose, firethorn and grape seeds using disc diffusion assay and spectrophotometric analysis on model organisms Staphylococcus aureus and Bacillus subtilis. Then I applied the extracts to meat samples and analyzed their effects on microbial growth.<\/em><\/span><\/p>\n

Professional Experiences and Internships<\/strong><\/span><\/p>\n

July-August 2018      Department of Physical Microfluidics and Bioengineering, <\/span>Institut Pasteur, Paris, France, <\/span>Erasmus Internship<\/em><\/span><\/p>\n

Achievements: I worked on making collagen, fibrin and GelMA gels, I learned soft lithography chip fabrication and carried out 3D cell culture by encapsulating HUVEC, 3T3 and C2C12 cell lines in collagen and fibrin gels both conventionally and by forming droplets inside chips, and followed the migration of cells in 3D through live cell imaging<\/em><\/span><\/p>\n

July-August 2017     Department of Bacterial Genome Plasticity, Institut Pasteur, Paris, France, <\/span>Erasmus Internship<\/em><\/span><\/p>\n

Achievements: <\/em>Protein characterization through construction of mutants and doing viability studies under DNA damaging conditions to study the SOS induction in Vibrio cholera. Doing pathogenic bacteria culture, various PCR applications, competent cell preparation, electroporation, transformation, growth curve analysis and sample preparation for FACS analysis<\/em><\/span><\/p>\n

August 2016             Department of Genomics, <\/span>Izmir International Biomedicine and Genome Institute, Turkey. <\/strong><\/span><\/p>\n

Achievements: Optimization of Gateway cloning, gel purification<\/em><\/span><\/p>\n

July 2016                   Department of Microbiology and Quality Assurance, <\/span>Pinar Integrated Meat Company                        <\/strong><\/span><\/p>\n

Achievements: Microbiological analysis on meat products (Listeria monocytogenes, Salmonella, Escherichia coli, Lactobacilli, mold and yeast analyses), doing pathogenic bacteria culture, qPCR<\/em> analyses<\/em><\/span><\/p>\n

July 2015                  Department of Molecular Biology and Genetics, <\/span>GENMAR Diagnostic Company<\/span><\/p>\n

Achievements: PCR primer synthesis, PCR applications and Sanger\/Next Generation Sequencing<\/em><\/span><\/p>\n

2012                       Student Science Projects Team, <\/span>Izmir Private \u00c7amlaralt\u0131 High School<\/span><\/p>\n

Achievements: Conduction of the project \u201cMeasuring the Avidity of Monoclonal Antibodies Produced Against Toxoplasma gondii with ELISA using NaSCN\u201d. Doing Enzyme Linked Immunosorbent Assay (ELISA) studies, result analysis<\/em><\/span><\/p>\n

2012                          Student Science Projects Team, <\/span>Izmir Private \u00c7amlaralt\u0131 High School<\/span><\/p>\n

Achievements: Conduction of the project \u201cBiosorption of Pb+2<\/sup> and Cd+2<\/sup> Ions from Aqueous Solutions Using Phaeodactylum tricornutum, Chlorella vulgaris and Scenedesmus dimorphus\u201d. Carrying out microalgae culture, biosorption experiments, FTIR measurements, applying the data to isotherms <\/em><\/span><\/p>\n

2011                        Student Science Projects Team, <\/span>Izmir Private \u00c7amlaralt\u0131 High School<\/span><\/p>\n

Achievements: Conduction of the project \u201cThe Antibacterial Effect of Montmorillonite Modified with Ag+1<\/sup>\u201d. Adsorption, Volhard Titration and disc diffusion experiments, <\/em>data interpretation<\/em><\/span><\/p>\n\n\n\n\n\n\n
Professional Experiences and Internships<\/strong><\/span><\/td>\n<\/tr>\n
<\/td>\nLaboratory Skills<\/strong><\/span><\/p>\n

 <\/strong><\/span><\/td>\n

Bioengineering:<\/strong> Design and fabrication of microfluidic systems, photolithograpy, softlithography<\/span><\/p>\n

Biomaterials:<\/strong> Synthesis of photocrosslinkable hydrogels, 3D cell culture systems using fibrin, GelMA, PEG, collagen\u2026<\/span><\/p>\n

Cell Culture:<\/strong> Differentiation and protocol development for iPSCs, 3D cell culture under both static and dynamic micro environments\/devices, culture of primary mammalian cells, cell lines, bacteria and microalgae<\/span><\/p>\n

Genetic and Molecular Biology:<\/strong> Plasmid transformation, Nucleic acid isolation, PCR primer design and synthesis, qPCR, ELISA<\/span><\/p>\n

Imaging and Histology:<\/strong> Live cell imaging, Confocal microscopy (Zeiss LSM 880), ICC, IHC<\/span><\/p>\n

Lab animals:<\/strong> Certified user of mice and rats<\/span><\/p>\n

Chemistry: <\/strong>Adsorption\/biosorption, titration, FTIR<\/span><\/td>\n

<\/td>\n<\/tr>\n
<\/td>\nComputer Skills<\/strong><\/span><\/td>\nMS Office (Word, PowerPoint, Excel)<\/span><\/p>\n

GraphPad<\/span><\/p>\n

CorelDraw<\/span><\/p>\n

Adobe Photoshop<\/span><\/p>\n

Matlab (beginner)<\/span><\/td>\n

<\/td>\n<\/tr>\n
<\/td>\n<\/td>\n<\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n\n\n\n\n\n\n\n
Scholarships and Awards<\/strong><\/span><\/td>\n<\/tr>\n
2018<\/span><\/td>\n3rd Place in the Best Poster Award Biomedical Science and Technology Symposium<\/span><\/td>\n<\/td>\n<\/tr>\n
2018<\/span><\/td>\nScholarship for Outstanding Master\u2019s Students, The Scientific and Technological Research Council of Turkey<\/span><\/td>\n<\/td>\n<\/tr>\n
2017<\/span><\/td>\n\u2018The Effect of Thyme Oil to Stem Cell Therapy in Experimental Wound Healing Model\u2019, 3rd<\/sup> Place in the Engineering Category of Manisa Celal Bayar University 24th Science and Spring Festival Science Projects Contest<\/span><\/td>\n<\/td>\n<\/tr>\n
2017<\/span><\/td>\n\u2018The Effect of Mesenchymal Stem Cells and Thyme Oil in Wound Healing\u2019 Undergrad Research Project Funding, The Scientific and Technological Research Council of Turkey<\/span><\/td>\n<\/td>\n<\/tr>\n
2012<\/span><\/td>\n\u2018Measuring the Avidity of mAbs Produced Against Toxoplasma gondii<\/em> with ELISA Using NASCN\u2019 Finalist of Acibadem University Project Olympiad, 2012<\/span><\/td>\n<\/td>\n<\/tr>\n
2012<\/span><\/td>\n\u2018Biosorption of Pb+2<\/sup> And Cd+2<\/sup> Ions From Aqueous Solutions Using Phaeodactylum  Tricornutum, Chlorella Vulgaris<\/em> and Scenedesmus Dimorphus<\/em>\u2019 Honorable Mention, International Sustainable World Energy Engineering Environment Project Olympiad, Houston, TX<\/span><\/td>\n<\/td>\n<\/tr>\n
2011<\/span><\/td>\n\u2018The Antibacterial Effect Of Montmorillonite Modified With Ag+1<\/sup>\u2018 The Best Project of 13th Expo Sciences International, Bratislava, Slovakia<\/span><\/td>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n\n\n
<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Publications<\/strong><\/span><\/p>\n

Asal, M.<\/strong>, G\u00fcven, S. (2019) \u2018Stem Cells: Sources, Properties and Cell Types\u2019, Vrana, N., Knopf-Marques, H., Barthes, J. (ed.) Biomaterials for Organ and Tissue Regeneration: New Technologies and Future Prospects<\/em>. Elsevier [book chapter submitted]<\/span><\/p>\n

Asal, M., \u00d6zen,<\/strong> \u00d6., \u015eahinler, M., Baysal, H. and Polato\u011flu, \u0130. (2018). An Overview of Biomolecules, Immobilization Methods and Support Materials of Biosensors. Sensor Review<\/em>, 39(3), pp. 377-386.<\/span><\/h1>\n

Asal, M.<\/strong>, \u00d6zen, \u00d6., \u015eahinler, M., Polato\u011flu, \u0130. (2018). Recent Developments in Enzyme, DNA and Immuno-Based Biosensors. Sensors (Basel), <\/em>18(6).<\/span><\/p>\n

Erbay, \u0130., Ba\u015f, A., Asal, M.<\/strong>, Yan\u0131k, H., G\u00fcven, S., (2018). \u2018Microfluidic Systems and Tissue Engineering\u2019, Kuralay, F. (ed.) Advanced Materials: Biomaterial and Nanomaterial Applications<\/em>. Palme Yay\u0131nlar\u0131 (in Turkish)<\/span><\/p>\n

International Conference Proceedings<\/strong><\/span><\/p>\n

Asal, M.<\/strong>, Y\u0131ld\u0131r\u0131m, C.A., G\u00fcven, S. \u2018Induced Pluripotent Stem Cells (iPSC) Derived Lacrimal Gland\u2019 24th International Biomedical Science and Technology Symposium, \u0130zmir, 17-20 October 2019<\/span><\/p>\n

Ba\u015f, A.K., Asal, M.<\/strong>, Kasal., K., Akbulud, O.B., Y\u0131ld\u0131r\u0131m, C.A., G\u00fcven, S. \u2018Bioengineering of Lacrimal Gland in Microfludics\u2019 24th International Biomedical Science and Technology Symposium, \u0130zmir, 17-20 October 2019<\/span><\/p>\n

S\u00f6nmez K\u0131l\u0131\u00e7arslan P., Toros P., Asal M.<\/strong>, \u015eahinler M., Tu\u011flu., \u0130. M. \u2018Effects of stem cells and thyme oil in rat wound healing models\u2019 2nd International Graduate Education Congress, Manisa, 12-14 May 2017 (in Turkish)<\/span><\/p>\n

\u00d6zen \u00d6., Baysal H.T., Asal M.<\/strong>, \u015eahinler M. \u2018The in vitro Effects of Stem Cells and Thyme Oil in Wound Healing Models in L929 Cell Line\u2019 2nd Life Sciences Congress, Kayseri, 23-25 February 2017<\/span><\/p>\n

National Conference Proceedings<\/strong><\/span><\/p>\n

Ba\u015f, A.K., Asal, M.<\/strong>, Kasal., K., Akbulud, O.B., Yan\u0131k, H., Y\u0131ld\u0131r\u0131m, C.A., G\u00fcven, S. 2018 \u2018Lacrimal Tissue Engineering\u2019 23rd Biomedical Science and Technology Symposium, \u0130stanbul, 15-16 December (in Turkish)<\/span><\/p>\n

Ba\u015f, A.K., Asal, M.<\/strong>, Kasal., K., Akbulud, O.B., Yan\u0131k, H., Y\u0131ld\u0131r\u0131m, C.A., G\u00fcven, S. 2018 \u2018Development of Lacrimal Gland From Stem Cells Using the Tissue Engineering Approach\u2019 5th Biomaterial Days, \u0130stanbul, 22-23 October (in Turkish)<\/span><\/p>\n

Certificates<\/strong><\/span><\/p>\n

Certificate of Experimental Animal Use (80 hours)<\/span><\/p>\n

Therapeutic mAb Engineering and Production Workshop (10 hours)<\/span><\/p>\n

Extra-Curricular Activities<\/strong><\/span><\/p>\n

2018-\u2026              Dokuz Eyl\u00fcl University, Faculty of Fine Arts, Department of Stage Arts, Acting<\/span><\/p>\n

2018-\u2026              Yol Sahne Theatre Troupe, Acting and Dramaturgy<\/span><\/p>\n

2016-2018         Muradiye Campus Theater Club, Management and Membership<\/span><\/p>\n

2016-\u2026              L\u00d6SEV (Children with Leukemia Foundation) Volunteer<\/span><\/p>\n

2016-2018         Izmir Tarz Dance Academy, Classical Ballet Student<\/span><\/p>\n

2015-2017         Izmir Tango Academy, Argentine Tango Student<\/span><\/p>\n

Languages<\/strong><\/span><\/p>\n

Turkish (Native)<\/span><\/p>\n

English (Excellent): IELTS Score 8<\/span><\/p>\n

German (Beginner)<\/span><\/p>\n

Spanish (Beginner)<\/span><\/p>\n","protected":false},"excerpt":{"rendered":"

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